Single molecule biophysics and poisson process approach to statistical mechanics /

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Single molecule biophysics and poisson process approach to statistical mechanics /

Sarkar, Susanta K., - Personal Name; Institute of Physics (Great Britain), - Personal Name; Morgan & Claypool Publishers, - Personal Name;

"Version: 20160401"--Title page verso."A Morgan & Claypool publication as part of IOP Concise Physics"--Title page verso.Includes bibliographical references.Preface -- 1. Single molecule biophysics -- 1.1. Introduction -- 1.2. A brief history of single molecule study -- 1.3. Single molecule methods -- 1.4. Single molecule data analysis -- 1.5. Brief overview of thermodynamics and statistical mechanics -- 1.6. Future outlook -- 1.7. Conclusion2. The total internal reflection fluorescence microscope -- 2.1. Introduction -- 2.2. Design considerations -- 2.3. Microscope frame and associated parts -- 2.4. Microscope objectives -- 2.5. Detection path and camera -- 2.6. Excitation path -- 2.7. Computer, computer software, and other computing hardware -- 2.8. Experimental protocol for slide preparation -- 2.9. Specifications of the TIRF microscope -- 2.10. Objective-type TIRFM -- 2.11. Multichannel detection and FRET -- 2.12. Circular dichroism spectroscopy and microscope enclosure -- 2.13. Acknowledgments3. Poisson process approach to statistical mechanics -- 3.1. Introduction -- 3.2. Diffusion -- 3.3. Inter-conversion of the states of molecules -- 3.4. Photon statistics from lasers -- 3.5. Statistics of fluorescent centers on a surface -- 3.6. Location of stars in the sky -- 3.7. Quantifying the heterogeneity of the locations of cosmic ray sources -- 3.8. Conclusion -- 3.9. AcknowledgmentsAppendices. -- A. Building a total internal reflection fluorescence microscope: parts, vendors, and costs -- B. The Poisson process.This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.Also available in print.Mode of access: World Wide Web.System requirements: Adobe Acrobat Reader.Dr. Susanta Sarkar is an Assistant Professor and Principal Investigator of the Single Molecule Biophysics Lab at the Colorado School of Mines. He studies biomolecules and nanoparticle one by one to understand their functions and properties. He received his PhD at the University of Oregon, completed his postdoctoral study at Cornell, and worked at the National Institutes of Health before joining the School of Mines.Title from PDF title page (viewed on May 6, 2016).

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Series Title: -
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Publisher: : .,
Collation: 1 online resource (various pagings) :illustrations (some color).
Language: English
ISBN/ISSN: 9781681741161
Classification: 571.4
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Carrier Type: -
Edition: -
Subject(s): Biophysics.
SCIENCE / Life Sciences / Biophysics.
TECHNOLOGY & ENGINEERING / Measurement.
Biophysics
Biomolecules
Instruments and instrumentation engineering.
Biomolecules.
Molecular Biology
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Statement of Responsibility: Susanta K. Sarkar.

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