Physics of cancer.
"Version: 20231101"--Title page verso.Includes bibliographical references.part I. Magnetics-based techniques from a physics of cancer perspective. 1. Examination of cancer cells and their ambient matrix using NMR and MRE -- 1.1. The principles of, and a short introduction to nuclear magnetic resonance -- 1.2. An overview of in-cell NMR techniques from prokaryotic to eukaryotic cells -- 1.3. A foray into the structures of molecules with an emphasis on cells -- 1.4. The primary emphasis is on structures, such as living cells, in their physical environment -- 1.5. In-cell NMR with an emphasis on mammalian cell analysis -- 1.6. A major measurement challenge for biological substances -- 1.7. The use of NMR in the field of cancer research -- 1.8. The applicability of NMR to living cells and (bio)polymeric matrices -- 1.9. An introduction to the bulk mechanical properties of cells, cell clusters, tissues, and extracellular matrices -- 1.10. Examining cell cluster and matrix mechanics using magnetic resonance elastography -- 1.11. Applications of MRE in cancer research -- 1.12. Conclusions and future directions2. Magnetic tweezer principles, results, and applications in cancer -- 2.1. An introduction to the magnetic tweezer technique -- 2.2. The evolution of vertical magnetic tweezers -- 2.3. The evolution of horizontal magnetic tweezers -- 2.4. Selected features of magnetic tweezers -- 2.5. Measurements of subcellular components and cells -- 2.6. Measuring matrix mechanical properties using beads -- 2.7. Advancements in magnetic tweezers -- 2.8. The effect of the direction of cell probing carried out using a rotating magnetic tweezer -- 2.9. The use of multipole magnetic tweezers to perform intracellular assessment of nuclear stiffening over time -- 2.10. A discussion of the weaknesses and strengths of magnetic tweezers -- 2.11. The future direction of magnetic tweezers3. Magnetic twisting cytometry of cancer cells -- 3.1. A short historic introduction to magnetic twisting cytometry -- 3.2. Overview of the process -- 3.3. Comparison with other techniques -- 3.4. Applications of the 3D MTC technique -- 3.5. Combining 3D MTC and confocal microscopy -- 3.6. Magnetic transduction -- 3.7. The benefits and drawbacks of employing magnetic forces -- 3.8. Magnet-based and directed 3D cell aggregation -- 3.9. Magnetic levitation using a dual magnet -- 3.10. Magnetic techniques used to examine cells and their ambient surroundings -- 3.11. Determining the dynamic mechanotransduction characteristics -- 3.12. Conclusions and future directionspart II. Laser-based techniques from a cancer-specific perspective. 4. Cytoskeletal remodeling dynamics of single normal and cancerous cells from a biological viewpoint -- 4.1. An introduction to the cytoskeletal remodeling of normal and cancerous cells -- 4.2. The cytoskeletons of normal and cancerous cells -- 4.3. The function of actin-binding proteins -- 4.4. Actin serves as a matrix for force generation -- 4.5. Microtubules and the tau protein -- 4.6. Intermediate filaments -- 4.7. Conclusions and future research5. Cytoskeletal remodeling dynamics of single normal and cancerous cells from a physical viewpoint -- 5.1. A short overview of nanoscale particle tracking techniques used to detect remodeling in cells -- 5.2. The physical principles of particle tracking -- 5.3. Understanding biophysical techniques for particle tracking in cells -- 5.4. Ensemble dynamic approaches -- 5.5. Particle tracking approaches -- 5.6. A brief overview of 3D particle tracking approaches -- 5.7. Instrumentation requirements for particle tracking approaches -- 5.8. Specimen requirements -- 5.9. Image quality -- 5.10. The tracking approach and linking tracks -- 5.11. Analysis of particle tracking -- 5.12. Combining individual molecule tracking inside living cells with localization microscopy of bioorthogonally tagged membrane proteins -- 5.13. Diffusion state shifts in single-particle trajectories of the mesenchymal-epithelial transition receptor tyrosine kinase in living cells -- 5.14. Real-time 3D single-particle tracking in living cells -- 5.15. Three-dimensional tracking techniques that provide active feedback -- 5.16. A comparative discussion of the various methods -- 5.17. Outlook -- 5.18. Conclusions and future research6. Optical tweezers -- 6.1. A historical introduction to the optical tweezer -- 6.2. The theory and fundamental principle of optical trapping -- 6.3. Brownian motion in optical tweezers -- 6.4. Experimental setups -- 6.5. Single living blood cell mechanical measurements -- 6.6. Holographic optical tweezers -- 6.7. Single-cell mechanics and molecular forces -- 6.8. Cancer-cell-associated mechanics -- 6.9. Individual cell analysis using optical tweezers inside cell crowds -- 6.10. The weaknesses, strengths, and future advancements of optical tweezers -- 6.11. Conclusions and future perspectives on optical tweezers7. Optical cell stretchers and their findings for cancer-specific issues -- 7.1. The historic background of the optical cell stretcher development -- 7.2. A comprehensive introduction to measurements with the optical cell stretcher -- 7.3. Key goals of the optical cell stretching technique -- 7.4. The working principles of the optical cell stretcher -- 7.5. Characterizing cell deformation during optical cell stretching -- 7.6. The effects of internal cellular and external device-dependent parameters on cellular deformability analyses -- 7.7. Strengths of the optical cell stretcher technique -- 7.8. Weaknesses of the optical cell stretching technique -- 7.9. The identification of gaps in the assessment of cell mechanical properties using the optical cell stretching technique -- 7.10. Modifications of the optical cell stretching procedure -- 7.11. An advanced biophysical optical stretching tool : the optical cell rotator -- 7.12. The future role of the optical cell stretcher -- 7.13. Cancer-specific applications : cancer disease detection for diagnosis, prognosis, and treatment -- 7.14. The combination of optical cell stretching with other cell biological techniques -- 7.15. Concluding remarks and outlook8. High-throughput microfluidics-based cell stretching -- 8.1. An introduction to microfluidics-based cell stretching -- 8.2. A comparison between constriction-based deformability cytometry, shear flow deformability cytometry, and extensional flow deformability cytometry -- 8.3. An overview of the different actuation (pressurization) techniques -- 8.4. Shear flow deformability cytometry techniques -- 8.5. An overview of examination (sensing) techniques -- 8.6. Real-time measurements -- 8.7. Sorting or separation procedures -- 8.8. Advances in optical cell stretching -- 8.9. A discussion of the weaknesses and strengths of microfluidics-based high-throughput techniques for mechanical analysis -- 8.10. Future applications of microfluidic techniques based on cellular mechanical properties -- 8.11. Perspectives and future directions.This is the fifth volume of the highly regarded Physics of Cancer (Second Edition) series, written with the aim of making very important topics in the physics of cancer visible to the research community. This fifth volume deals with biophysical methods. The first chapter presents the traditional NMR techniques and introduces the relatively new MRE technique for the characterization of cancer cells and tissues. The second chapter provides an overview of magnetic tweezer techniques relevant to cell characterization. The third chapter focusses on the magnetic twisting cytometry techniques. The fourth and fifth chapters discuss the cytoskeletal remodeling dynamics from a biological viewpoint and a physical viewpoint, respectively. The optical tweezer is introduced in the sixth chapter and the optical cell stretcher is discussed in the seventh chapter. In the eighth chapter, microfluidics-based high-throughput cell stretching is discussed and the latest developments are presented. Part of Biophysical Society-IOP series.Scientists, researchers, graduate students, instructors in biophysics.Also available in print.Mode of access: World Wide Web.System requirements: Adobe Acrobat Reader, EPUB reader, or Kindle reader.Claudia Tanja Mierke is Head of the Department of Biological Physics at the Peter Debye Institute for Soft Matter Physics at Leipzig University. Her primary research areas are cell biophysics and cell mechanics, adhesion, motility (invasion) in biomimetic matrices, cancer and inflammation, cancer metastasis and tumormicroenvironment mechanics. She has published over 150 referred journal articles, books and book chapters largely dealing with soft matter physics and the physics of cancer. Over the past 18 years, Claudia had taught courses in biophysics, soft matter physics, cell biology for physicists and cellular biophysics, to both undergraduate and graduate students.Title from PDF title page (viewed on January 4, 2024).
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1 online resource (various pagings) :illustrations (some color).
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English
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9780750362740
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616.994
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Second edition.
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Claudia Tanja Mierke.
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